Question:
You’re transitioning from using a Lynx to the myQA Phoenix and Sphinx Compact. How can you determine if your image is a good image?
Answer:
For Phoenix and Sphinx Compact: Focus on Count Limits
- The key indicator for a good image is count limit, not image intensity.
- Refer to the count limit specifics for your devices in the detailed guide: Phoenix Sampling Frequency Article.
For Lynx (Historical Approach): Lynx Range
- In myQA FastTrack, images were scaled according to original intensity in the Lynx Range.
- Signal thresholds were 30% minimum (under-irradiation) and 90% maximum (over-irradiation).
- Adjustments included modifying the iris or MU per spot to address saturation or low signal.
- For detailed guidance, see: Recommended Intensity Exposure of Lynx.
Phoenix and Sphinx Compact: Revised Process
- These devices have a larger original intensity range due to the ability to change capacitors via gain values.
- Images are displayed in Image Max; Lynx Range isn’t available.
- Thresholds for 90% and 30% intensity are less relevant. Focus on counts instead of intensity.
Steps for Phoenix & Sphinx Compact:
1. Focus on Total Counts:
- Adjust the gain values to address saturation or low SNR.
- Refer to the guide for selecting the gain based on energy and MU/Spot: Gain Value Selection Guide.
2. Check Saturation Warnings:
- After capturing an image, the software will display a yellow triangle on the measurement if saturation occurs.
3. Analyze Total Counts:
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Profile Tab:
- In myQA FastTrack, go to Profile > Analysis.
- Change Normalization to "Raw Data (cnt)" for spot profiles.
-
Multiple Spot Analysis:
- Analyze counts using both 1-D Gaussian Fit and 2-D Gaussian Fit.
4. Count Values Per Frame:
- Ensure counts don’t exceed 65536 counts per frame.
- Locate measurement settings in the bottom-right corner of myQA FastTrack after capturing spots.
Additional Reading:
- Spot Size & Position Relationship: Spot Size and Position.
- Count Number and Gain Value: Gain Value and Counts.